Reaction Details  Report a problem with these data
Cell Reactant:
Heat Shock Protein 90 (Hsp90)
Syringe Reactant:
BDBM31995
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2009-09-29
ΔG°:
-6.53±n/a (kcal/mole)
pH:
8.0000±n/a
Log10Kb:
3.8
Temperature:
288.1500±n/a (K)
ΔHobs :
-17.99±0.36 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
-0.04±n/a (kJ/mole-K)
Citation
 Nilapwar, S; Williams, E; Fu, C; Prodromou, C; Pearl, LH; Williams, MA; Ladbury, JE Structural-thermodynamic relationships of interactions in the N-terminal ATP-binding domain of Hsp90. J Mol Biol 392:923-36 (2009) [PubMed]  Article
Cell React
Source:
His-tagged N-terminal domain of yeast HSP90 was expressed and purified.
Prep. Method:
Protein was purified by using nickel affinity column, and was subsequently concentrated by an (NH4)2SO4 precipitation and purified further using gel filtration chromatography.
Name:
Heat Shock Protein 90 (Hsp90)
Synonyms:
HSP82_YEAST | HSP82 | HSP90 | 82 kDa heat shock protein | ATP-dependent molecular chaperone HSP82 | Heat shock protein Hsp90 heat-inducible isoform
Type:
N-terminal domain
Mol. Mass.:
23389.88
Organism:
Baker's yeast
Description:
P02829 aa 1-207
Residue:
207
Sequence:
MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQL
  
Syringe React
Name:
BDBM31995
Synonyms:
ADP | CHEMBL14830 | Adenosine Diphosphate (ADP)
Type:
Nucleoside or nucleotide
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
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