Reaction Details  Report a problem with these data
Cell Reactant:
Quinone Reductase 2 (QR2)
Syringe Reactant:
BDBM29611
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2009-06-10
ΔG°:
-8.5±0.38 (kcal/mole)
pH:
7.5000±n/a
Log10Kb:
5.7
Temperature:
298.1500±n/a (K)
ΔHobs :
-7.85±0.62 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.8000
ΔS° :
0±0 (kJ/mole-K)
Citation
 Calamini, B; Santarsiero, BD; Boutin, JA; Mesecar, AD Kinetic, thermodynamic and X-ray structural insights into the interaction of melatonin and analogues with quinone reductase 2. Biochem J 413:81-91 (2008) [PubMed]  Article
Cell React
Source:
Human QR2 was expressed and purified from E. coli cells.
Prep. Method:
The enzyme was further applied to a Hiload 26/60 Superdex 75 (GE Healthcare) column. The fractions containing pure enzyme were pooled and finally applied to a 10/100 GL MonoQ (GE Healthcare) strong anion-exchange column. The protein was eluted using a gradient. The purest protein fractions were pooled, buffer-exchanged and concentrated. Samples of QR2 were extensively dialysed against ITC buffer, and degassed before assay.
Name:
Ribosyldihydronicotinamide dehydrogenase [quinone]
Synonyms:
QR2 | Quinone reductase 2 (NQO2) | Metallothionein-3 | NRH:quinone oxidoreductase 2 | Ribosyldihydronicotinamide dehydrogenase [quinone] | NRH dehydrogenase [quinone] 2 | Quinone reductase 2 | NQO2_HUMAN | NQO2 | NMOR2
Type:
Protein
Mol. Mass.:
25917.25
Organism:
Human
Description:
P16083
Residue:
231
Sequence:
MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNLEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ
  
Syringe React
Name:
BDBM29611
Synonyms:
CHEMBL289233 | Melatonin,2-Iodo | 2-Iodomelatonin
Type:
Small organic molecule
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: