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Cell Reactant:
PLD alpha C2
Syringe Reactant:
BDBM14
Meas. Tech.:
ITC and CD
Entry Date.:
2000-12-04
ΔG°:
-4.62±n/a (kcal/mole)
pH:
7.5000±n/a
Temperature:
303.1500±n/a (K)
ΔH° :
6.53±n/a (kJ/mole)
ΔHobs :
6.53±n/a (kJ/mole)
ΔS° :
0.04±n/a (kJ/mole-K)
Citation
 Zheng, L; Krishnamoorthi, R; Zolkiewski, M; Wang, X Distinct Ca2+ binding properties of novel C2 domains of plant phospholipase dalpha and beta. J Biol Chem 275:19700-6 (2000) [PubMed]  Article
Cell React
Source:
poly-His-fused C2 proteins were expressed in E. coli BL21(DE3)
Prep. Method:
C2 proteins without the tag were refolded by passing through a Sephacryl S-100 gel filtration column.Proteins were dissolved in Chelex-100 treated buffer
Name:
PLD alpha C2
Synonyms:
PLD alpha C2 domain | PLDA2_ARATH | PLDALPHA2 | PLD2
Type:
Protein
Mol. Mass.:
91590.93
Organism:
Mouse-ear cress
Description:
Q9SSQ9
Residue:
810
Sequence:
MEECLLHGRLHATIYEVDHLHAEGGRSGFLGSILANVEETIGVGKGETQLYATIDLEKARVGRTRKITKEPKNPKWFESFHIYCGHMAKHVIFTVKDANPIGATLIGRGYIPVEDILHGEEVDRWVDILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSAKFPGVPYTFFSQRRGCKVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKHLIYITGWSVYTEISLVRDSRRPKQGGDVTVGELLKKKASEGVKVILLVWDDRTSVDLLKKDGLMATHDEETENFFRGTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEMPSGGSRSRRIVSFVGGLDLCDGRYDTPFHSLFRTLDTAHHDDFHQPNFTGAAITKGGPREPWHDIHCRLEGPIAWDVLYNFEQRWSRQGGKDILVKMRELGDIIIPPSPVLFSEDHDVWNVQLFRSIDGGAAAGFPDSPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYIENQYFLGSSFAWSADGIKPEEINALHLIPKELSLKIVSKIKAGEKFKVYVVVPMWPEGIPESGSVQAILDWQKRTMEMMYKDVIKALRENGLEGEDPRDYLTFFCLGNREVKKDGEYEPSEKPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLSTRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECIQKVNRVADKYWDLYSSESLEHDLPGHLLRYPIGIASEGNITELPGCEFFPDTKARILGVKSDYMPPILTT
  
Syringe React
Source:
Fisher Scientific
Name:
BDBM14
Synonyms:
calcium | Ca2+
Type:
Metal Ion
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: