Reaction Details
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Cell Reactant:
Major Urinary Protein (MUP-1)
Syringe Reactant:
BDBM22603
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2008-06-15
ΔG°:
-5.53±0.02 (kcal/mole)
pH:
7.4000±n/a
Temperature:
300.0000±n/a (K)
ΔHobs :
-9.81±0.77 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
1.1000
ΔS° :
-0.01±0 (kJ/mole-K)
Citation

Cell React
Source:
The MUP-1 gene was cloned and expressed in E. coli cells.
Prep. Method:
MUP-I was purified using Ni-NTA resin. To remove endogenous bound ligands, an ethanol precipitation step was utilized involving addition of 2 volumes of absolute ethanol per volume of protein solution, followed by incubation at 4 °C for 2 h. After centrifugation at 5000g, the pellet was lyophilised, resuspended in PBS pH 7.4, and dialyzed overnight against the same buffer.
Name:
Major Urinary Protein (MUP-1)
Synonyms:
Major urinary protein 1 precursor | MUP1_MOUSE | Mup1
Type:
Other Protein Type
Mol. Mass.:
20640.17
Organism:
Mouse
Description:
n/a
Residue:
180
Sequence:
MKMLLLLCLGLTLVCVHAEEASSTGRNFNVEKINGEWHTIILASDKREKIEDNGNFRLFLEQIHVLENSLVLKFHTVRDEECSELSMVADKTEKAGEYSVTYDGFNTFTIPKTDYDNFLMAHLINEKDGETFQLMGLYGREPDLSSDIKERFAQLCEKHGILRENIIDLSNANRCLQARE