Reaction Details  Report a problem with these data
Cell Reactant:
BirA Bifunctional Protein Mutant (V214A)
Syringe Reactant:
BDBM12
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2007-12-09
ΔG°:
-9.9±0.1 (kcal/mole)
pH:
7.5000±0.0200
Log10Kb:
6.7
Temperature:
293.1500±0.1000 (K)
ΔHobs :
-19.5±0.2 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
0.8500
ΔS° :
-0.03±0 (kJ/mole-K)
Citation
 Naganathan, S; Beckett, D Nucleation of an allosteric response via ligand-induced loop folding. J Mol Biol 373:96-111 (2007) [PubMed]  Article
Cell React
Source:
The BirA variants were expressed in the Escherichia coli strain JM109 transformed with the plasmid pBtac2 carrying the E. coli birA gene (wild-type or mutant) under transcriptional control of the tac.
Purity:
>95%
Prep. Method:
The separation and purification of BirA from other cellular proteins was carried out in NiNTA column and Sepharose chromatography steps.
Name:
BirA Bifunctional Protein Mutant (V214A)
Synonyms:
BirA adenylate-binding loop (ABL) variant
Type:
Repressor variant
Mol. Mass.:
35285.53
Organism:
Escherichia coli
Description:
n/a
Residue:
321
Sequence:
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRAEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
  
Syringe React
Source:
Sigma-Aldrich
Name:
BDBM12
Synonyms:
cid_171548 | cid_253 | 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid | Biotin
Type:
Enzyme co-factor
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: