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Cell Reactant:
tRNA synthetase (GlyRS)
Syringe Reactant:
BDBM18138
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2007-10-01
ΔG°:
-8.95±0.19 (kcal/mole)
pH:
7.2000±n/a
Log10Kb:
6
Temperature:
298.0000±n/a (K)
ΔHobs :
-44.74±2.27 (kJ/mole)
Corrected for ΔHioniz:
yes
Stoich. Param.:
1.0500
ΔS° :
-0.12±0.01 (kJ/mole-K)
Citation
 Dignam, JD; Nada, S; Chaires, JB Thermodynamic characterization of the binding of nucleotides to glycyl-tRNA synthetase. Biochemistry 42:5333-40 (2003) [PubMed]  Article
Cell React
Source:
Protein was expressed and purified from E. coli.
Prep. Method:
Enzyme was purified by affinity chromatography.
Name:
Glycine--tRNA ligase
Synonyms:
Glycine--tRNA ligase | Glycyl-tRNA synthetase | GARS_BOMMO | GlyRS | tRNA synthetase (GlyRS)
Type:
Enzyme
Mol. Mass.:
76917.70
Organism:
Silk moth
Description:
B. mori glycyltRNA synthetase was expressed in E. coli.
Residue:
680
Sequence:
MADPKIEEILAPLRANVKEQGDLVRKLKEEKAPEIDIKKAVAELKTRKKILEDKELSLAPAEDLFDRAKMEDLIKRRFFYDQSFAIYGGITGQFDFGPMGCALKSNMIHLWKKFFILQEQMLEVECSILTPEPVLKASGHVERFADLMTKDIKTGECFRLDHLIKGHLEKIKSDKNTKIELKAEIEDILIKLDGMNADEMSALMKRFEMKSPISGNDLTPPIEFNLMFNTQIGPSGLVKGFLRPETAQGIFVNFKRLLEFNQGRLPFAAAQIGNSFRNEISPRSGLLRVREFTMCEIEHFCDVKEHPKFESVKNTQSLLYSADNQEQGKPADLTTIGDAVCKGIVNNETLGYFMARIHMYMLAVGIDPKRLRFRQHMGNEMAHYACDCWDAECLSSYGWIECVGCADRSAYDLTQHTKATGIRLAAEKKLPAPKQIEVVEAIANNGRIGKAFKKDSQAINDTLATLDNAALEEMQKELDSNGEYTLITARGEFKLTPSLVNVKKTQKTIHVEEIIPSVIEPSFGVGRILYCILEHNFRMREGDEQRTYFSLPPTVAPMKCVVLPLSGNAEFQPFVRDLSQELITVDVSHKVDDSSGSIGRRYARTDELGVPYAVTVDFDTIKEPHTVTLRERDSMRQVRLPMADVPTVVRDLSNSKILWSDVEQKYPKFEQQETVKGTSV
  
Syringe React
Source:
Sigma-Aldrich
Name:
BDBM18138
Synonyms:
5-O-[N-(L-glycyl)sulfamoyl]adenosine | 2-amino-1-[({[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}sulfonyl)amino]ethan-1-one | Glycine sulfamoyl adenosine | GSAd
Type:
Nucleoside or nucleotide
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: