Reaction Details  Report a problem with these data
Cell Reactant:
Thymidine Kinase (TK.dT)
Syringe Reactant:
BDBM2
Meas. Tech.:
ITC
Entry Date.:
2000-12-13
ΔG°:
-9.14±0.35 (kcal/mole)
pH:
7.5000±n/a
Temperature:
293.1500±n/a (K)
ΔH° :
-13.06±0.65 (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
-0.3100
ΔCp :
-0.14±0.02 (kJ/mole)
ΔS° :
-0.01±0.01 (kJ/mole-K)
Citation
 Perozzo, R; Jelesarov, I; Bosshard, HR; Folkers, G; Scapozza, L Compulsory order of substrate binding to herpes simplex virus type 1 thymidine kinase. A calorimetric study. J Biol Chem 275:16139-45 (2000) [PubMed]  Article
Cell React
Purity:
More than 99% of the enzyme was present as TK.dT complex
Prep. Method:
0.0373 mM enzyme exists in the presence of 1 mM dT.
Name:
Thymidine Kinase (TK.dT)
Synonyms:
thymidine kinase and thymidine complex (TK.dT)
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Thymidine kinase
Synonyms:
KITH_HHV11 | TK
Type:
Enzyme
Mol. Mass.:
40978.57
Organism:
HHV-1
Description:
P0DTH5
Residue:
376
Sequence:
MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQKATEVRLEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGAAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN
  
Component 2
Name:
BDBM1
Synonyms:
thymidine | dT
Type:
Nucleoside or nucleotide
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity:
Syringe React
Source:
Sigma
Name:
BDBM2
Synonyms:
CHEMBL14249 | ({[({[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl)oxy](hydroxy)phosphoryl}oxy)phosphonic acid | Brimonidine ATP | ATP
Type:
Nucleoside or nucleotide
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: