Reaction Details  Report a problem with these data
Cell Reactant:
Thymidine Kinase
Syringe Reactant:
BDBM1
Meas. Tech.:
ITC
Entry Date.:
2000-12-13
ΔG°:
-7.13±0.35 (kcal/mole)
pH:
7.5000±n/a
Temperature:
293.1500±n/a (K)
ΔH° :
-17.48±0.87 (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
0.0000
ΔCp :
-0.36±0.02 (kJ/mole)
ΔS° :
-0.03±0.01 (kJ/mole-K)
Citation
 Perozzo, R; Jelesarov, I; Bosshard, HR; Folkers, G; Scapozza, L Compulsory order of substrate binding to herpes simplex virus type 1 thymidine kinase. A calorimetric study. J Biol Chem 275:16139-45 (2000) [PubMed]  Article
Cell React
Source:
Expressed as glutathione S-transferase fusion protein in E. coli.
Prep. Method:
Purified by glutathione affinity chromatography.
Name:
Thymidine kinase
Synonyms:
KITH_HHV11 | TK
Type:
Enzyme
Mol. Mass.:
40978.57
Organism:
HHV-1
Description:
P0DTH5
Residue:
376
Sequence:
MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQKATEVRLEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGAAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN
  
Syringe React
Source:
Sigma
Name:
BDBM1
Synonyms:
thymidine | dT
Type:
Nucleoside or nucleotide
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: