Reaction Details 
Report a problem with these data
Report a problem with these dataCell Reactant:
Enoyl-ACP Reductase (FabI) Mutant (F203L)
Syringe Reactant:
BDBM3912
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2005-04-13
ΔG°:
-38.9000±n/a (kJ/mole)
pH:
7.5000±n/a
Log10Kb:
9.1
Temperature:
298.1500±n/a (K)
ΔH° :
-59.1000±2.5000 (kJ/mole)
ΔHobs :
-59.1000±2.5000 (kJ/mole)
Corrected for ΔHioniz:
no
ΔCp :
-2.5100±0.4200 (kJ/mole)
Stoich. Param.:
0.4900
ΔS° :
-0.0680±n/a (kJ/mole-K)
Citation
Protasevich, II; Brouillette, CG; Snow, ME; Dunham, S; Rubin, JR; Gogliotti, R; Siegel, K Role of inhibitor aliphatic chain in the thermodynamics of inhibitor binding to Escherichia coli enoyl-ACP reductase and the Phe203Leu mutant: a proposed mechanism for drug resistance. Biochemistry 43:13380-9 (2004) [PubMed] ArticleCell React
Source:
The His-tagged FabI (F203L) was expressed and purified from E. coli
Purity:
Purity was not lower than 95%
Prep. Method:
The calorimetric titration of FabI(F203L) was conducted in the presence of 0.5 mM NAD+.
Name:
Enoyl-ACP Reductase (FabI) Mutant (F203L)
Synonyms:
n/a
Type:
Binary complex with a cofactor
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Enoyl-ACP Reductase (FabI) Mutant (F203L)
Synonyms:
NADPH-dependent enoyl-ACP reductase
Type:
Enzyme Subunit
Mol. Mass.:
27827.10
Organism:
Escherichia coli
Description:
n/a
Residue:
262
Sequence:
MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKLKGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDGDYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMGLAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDLRKMLAHCEAVTPIRRTVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK
Component 2
Name:
BDBM3909
Synonyms:
NAD+
Type:
Nucleoside or nucleotide
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Syringe React
Source:
Organic Synthesis
Name:
BDBM3912
Synonyms:
5-ethyl-2-(2-hydroxyphenoxy)phenol | PD202165
Type:
Small organic molecule
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
