Cell Reactant:

Syringe Reactant:

Meas. Tech.:

ITC

Entry Date.:

2000-11-29

ΔG°:

-19.4000±0.3360 (kJ/mole)

pH:

7.5000±n/a

Temperature:

293.1500±n/a (K)

ΔH° :

-8.1900±0.0840 (kJ/mole)

ΔH_obs :

-8.1900±n/a (kJ/mole)

Corrected for ΔH_ioniz:

yes

Protons Released:

0.0000

ΔS° :

0.0380±0.0010 (kJ/mole-K)

Citation

 McNemar, C; Snow, ME; Windsor, WT; Prongay, A; Mui, P; Zhang, R; Durkin, J; Le, HV; Weber, PC Thermodynamic and structural analysis of phosphotyrosine polypeptide binding to Grb2-SH2. Biochemistry 36:10006-14 (1997) [PubMed]  Article

More Info.:

Get all data from this article,  ITC RUN data,  Solution Info,  Instrument Info

Source:

DNA construct for the Grb2-SH2 domain was expressed in E.coli

Purity:

>98%

Prep. Method:

Mono-Q and Mono-S columns

Name:

Growth factor receptor-bound protein 2

Synonyms:

Grb2-SH2 | GRB2 adapter protein | Growth factor receptor-bound protein 2 | GRB2_HUMAN | GRB2 | ASH

Type:

Protein

Mol. Mass.:

25205.04

Organism:

Human

Description:

P62993

Residue:

217

Sequence:

MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Blast this sequence in BindingDB or PDB

  
Blast E-value cutoff:

Source:

AnaSpec Inc. (San Jose, CA)

Prep. Method:

Fmoc solid-phase peptide synthesis

Name:

pY317 N(+2)A

Synonyms:

n/a

Type:

Peptide

Mol. Mass.:

665.74

Organism:

n/a

Description:

alanine substitution of pY317, a phosphotyrosine polypeptide

Residue:

6

Sequence:

SYVAVQ