Reaction Details  Report a problem with these data
Cell Reactant:
Lysozyme (hen egg)
Syringe Reactant:
HyHEL-10 Fv Mutant (HD32A)
Meas. Tech.:
ITC and CD
Entry Date.:
2000-12-11
ΔG°:
-48.4900±1.0500 (kJ/mole)
pH:
7.2000±n/a
Log10Kb:
7.7
Temperature:
303.1500±n/a (K)
ΔH° :
-115.7900±n/a (kJ/mole)
ΔHobs :
-115.7900±n/a (kJ/mole)
ΔCp :
-2.2500±0.0500 (kJ/mole)
Stoich. Param.:
1.0000
ΔS° :
-0.2220±0.0060 (kJ/mole-K)
Citation
 Tsumoto, K; Ogasahara, K; Ueda, Y; Watanabe, K; Yutani, K; Kumagai, I Role of salt bridge formation in antigen-antibody interaction. Entropic contribution to the complex between hen egg white lysozyme and its monoclonal antibody HyHEL10. J Biol Chem 271:32612-6 (1996) [PubMed]  Article
Cell React
Source:
Seikagaku Kogyo, Inc. (Tokyo, Japan)
Name:
Lysozyme C
Synonyms:
hen egg lysozyme (HEL) | FvD1.3(VLW92H) | FvD1.3(VLW92F) | FvD1.3(VLW92V) | FvD1.3(VLW92D) | FvD1.3(VLW92S) | FvD1.3(VLW92wt) | FvD1.3(VLW92A) | Lysozyme (hen egg) | LYSC_CHICK | LYZ
Type:
Enzyme
Mol. Mass.:
16248.07
Organism:
Chicken
Description:
P00698
Residue:
147
Sequence:
MRSLLILVLCFLPLAALGKVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
  
Syringe React
Source:
Secretory expression in E. coli
Prep. Method:
Affinity chromatography purification procedures using HEL-Sepharose
Name:
HyHEL-10 Fv Mutant (HD32A)
Synonyms:
n/a
Type:
Antibody
Mol. Mass.:
12741.84
Organism:
Mouse
Description:
Mutant of anti-hen egg lysozyme antibody HyHEL-10.
Residue:
114
Sequence:
DVQLQESGPSLVKPSQTLSLTCSVTGDSITSAYWSWIRKFPGNRLEYMGYVSYSGSTYYNPSLKSRISITRDTSKNQYYLDLNSVTTEDTATYYCANWDGDYWGQGTLVTVSAA