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Cell Reactant:
ferricytochrome c peroxidase (CcP)
Syringe Reactant:
iso-1-cytochrome c (Cc)
Meas. Tech.:
ITC
Entry Date.:
2000-12-15
ΔG°:
-39.0600±1.6800 (kJ/mole)
pH:
6.0000±n/a
Temperature:
298.1500±n/a (K)
ΔH° :
-26.0400±0.4200 (kJ/mole)
ΔHobs :
-19.7400±n/a (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
0.6000
ΔCp :
-0.9072±0.2604 (kJ/mole)
Stoich. Param.:
1.0000
ΔS° :
0.0648±0.0060 (kJ/mole-K)
Comments:
test the effect of ionic strengh on binding
Citation
 Wang, X; Pielak, GJ Equilibrium thermodynamics of a physiologically-relevant heme-protein complex. Biochemistry 38:16876-81 (1999) [PubMed]  Article
Cell React
Source:
recombinant yeast CcP was expressed and purified from E.coli
Prep. Method:
CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes
Name:
Cytochrome c peroxidase, mitochondrial
Synonyms:
CCP1 | CCPR_YEAST
Type:
Enzyme
Mol. Mass.:
40351.45
Organism:
Saccharomyces cerevisiae
Description:
P00431
Residue:
361
Sequence:
MTTAVRLLPSLGRTAHKRSLYLFSAAAAAAAAATFAYSQSQKRSSSSPGGGSNHGWNNWGKAAALASTTPLVHVASVEKGRSYEDFQKVYNAIALKLREDDEYDNYIGYGPVLVRLAWHTSGTWDKHDNTGGSYGGTYRFKKEFNDPSNAGLQNGFKFLEPIHKEFPWISSGDLFSLGGVTAVQEMQGPKIPWRCGRVDTPEDTTPDNGRLPDADKDADYVRTFFQRLNMNDREVVALMGAHALGKTHLKNSGYEGPWGAANNVFTNEFYLNLLNEDWKLEKNDANNEQWDSKSGYMMLPTDYSLIQDPKYLSIVKEYANDQDKFFKDFSKAFEKLLENGITFPKDAPSPFIFKTLEEQGL
  
Syringe React
Source:
purified from yeast
Prep. Method:
CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes
Name:
iso-1-cytochrome c (Cc)
Synonyms:
CYC1
Type:
Protein
Mol. Mass.:
12191.93
Organism:
Saccharomyces cerevisiae
Description:
A6ZQ07
Residue:
109
Sequence:
MTEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGRHSGQAEGYSYTDANIKKNVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLITYLKKACE