Reaction Details  Report a problem with these data
Cell Reactant:
CAM-RNase A
Syringe Reactant:
SecB
Meas. Tech.:
ITC
Entry Date.:
2000-12-13
ΔG°:
-28.9800±n/a (kJ/mole)
pH:
7.4000±n/a
Temperature:
283.1500±n/a (K)
ΔH° :
-35.2800±n/a (kJ/mole)
ΔCp :
-1.7220±n/a (kJ/mole)
Stoich. Param.:
1.0000
ΔS° :
-0.0220±n/a (kJ/mole-K)
Comments:
discrepancies in the fluorescence and calorimetric-derived binding constants
Citation
 Panse, VG; Swaminathan, CP; Surolia, A; Varadarajan, R Thermodynamics of substrate binding to the chaperone SecB. Biochemistry 39:2420-7 (2000) [PubMed]  Article
Cell React
Name:
Ribonuclease pancreatic
Synonyms:
CAM-RNase A | RNAse A | RNAS1_BOVIN | RNASE1 | RNS1
Type:
Enzyme
Mol. Mass.:
16468.79
Organism:
Bovine
Description:
carboxamidomethylated RNase A
Residue:
150
Sequence:
MALKSLVLLSLLVLVLLLVRVQPSLGKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV
  
Syringe React
Source:
Expressed in E. coli strain BL21 (DE3)
Purity:
99%
Prep. Method:
Fast flow Q-Sepharose column, and then a Sephacryl S-200 gel filtration column
Name:
SecB
Synonyms:
n/a
Type:
Protein
Mol. Mass.:
17264.58
Organism:
Shigella flexneri serotype 5b (strain 8401)
Description:
Q0SYD5
Residue:
155
Sequence:
MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDLDTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYCPNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA