BDBM50241461 3,7-bis(dimethylamino)phenothiazin-5-ium chloride::Basic Blue 9::CHEMBL405110::Methylenblau::Methylene Blue anhydrous::Methylene blue::Methylene blue (10)::SAMPL5, CBC-G10::azul de metileno::bleu de methylene::cid_6099::methylthioninium chloride

SMILES CN(C)c1ccc2nc3ccc(cc3sc2c1)=[N+](C)C

InChI Key InChIKey=RBTBFTRPCNLSDE-UHFFFAOYSA-N

Data  1 KI  23 IC50  4 EC50  1 ITC

  Tab Delimited (TSV)   2D SDfile   Computed 3D by Vconf -m prep SDfile
Find this compound or compounds like it in BindingDB:
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Similarity at least:  must be >=0.5
Exact match

Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB

Found 28 hits for monomerid = 50241461   

TargetAmine oxidase [flavin-containing] B(Human)
North-West University

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 4.37E+3nMAssay Description:Inhibition of human recombinant MAOB using kynuramine substrate assessed as reduction in MAO-generated 4-hydroxyquinoline level by fluorescence spect...More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetMicrotubule-associated protein tau(Human)
Oakland University

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 3.10E+4nMAssay Description:Inhibition of human full length recombinant tau-441 expressed in Escherichia coli assessed as inhibition of heparin-induced protein aggregation by me...More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetMicrotubule-associated protein tau(Human)
Oakland University

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataEC50:  590nMAssay Description:Inhibition of tau aggregation (unknown origin)More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetMicrotubule-associated protein tau(Human)
Oakland University

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataEC50:  130nMAssay Description:Inhibition of tau-tau binding (unknown origin) assessed as increase in dissolved paired helical filamentsMore data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetAmine oxidase [flavin-containing] A(Human)
North-West University

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 70nMAssay Description:Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cell microsomes using kynuramine as substrate after 20 mins by flu...More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetCruzipain(Trypanosoma cruzi)
University of California San Francisco

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 5.50E+4nMAssay Description:Inhibition of Trypanosoma cruzi cruzaine preincubated for 5 mins before substrate addition by fluorescence assay in absence of Triton X-100More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetCruzipain(Trypanosoma cruzi)
University of California San Francisco

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 8.50E+4nMAssay Description:Inhibition of Trypanosoma cruzi cruzaine preincubated for 5 mins before substrate addition by fluorescence assay in presence of 0.01% Triton X-100More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetMicrotubule-associated protein tau(Human)
Oakland University

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.40E+3nMAssay Description:Inhibition of recombinant human TAU three-repeat microtubule-binding domain aggregation expressed in Escherichia coli after 16 hrs by thioflavin T fl...More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetDihydrolipoyl dehydrogenase(malaria parasite P. falciparum)
Biochemie-Zentrum Der Universit£T Heidelberg

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.00E+6nMpH: 7.3Assay Description:Inhibition of Plasmodium falciparum recombinant dihydrolipoamide dehydrogenase at pH 7.3More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetDihydrolipoyl dehydrogenase, mitochondrial(Pig)
Biochemie-Zentrum Der Universit£T Heidelberg

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.00E+6nMpH: 7.3Assay Description:Inhibition of pig recombinant dihydrolipoamide dehydrogenase at pH 7.3More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetThioredoxin reductase 1, cytoplasmic(Human)
Biochemie-Zentrum Der Universit£T Heidelberg

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 3.00E+4nMpH: 7.4Assay Description:Inhibition of human recombinant TrxR1 at pH 7.4More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetGlutathione reductase, mitochondrial(Human)
Biochemie-Zentrum Der Universit£T Heidelberg

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.64E+4nMpH: 6.9Assay Description:Inhibition of human recombinant glutathione reductase at pH 6.9More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetGlutathione reductase(Plasmodium falciparum (isolate 3D7))
Université

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 6.40E+3nMAssay Description:Inhibitory concentration against Plasmodium falciparum glutathione reductaseMore data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetGlutathione reductase, mitochondrial(Human)
Biochemie-Zentrum Der Universit£T Heidelberg

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.60E+4nMAssay Description:Inhibitory concentration against human glutathione reductaseMore data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetPOsterior Segregation(Caenorhabditis elegans)
Broad Institute

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataEC50:  1.87E+5nMAssay Description:Broad Institute: MLPCN maternal gene expression Project ID: 2024 Keywords: Zinc finger, C. elegans, maternal gene expression, RNA-protein interac...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetAmine oxidase [flavin-containing] A(Human)
North-West University

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 70nMAssay Description:Inhibition of human recombinant MAOA using kynuramine substrate assessed as reduction in MAO-generated 4-hydroxyquinoline level by fluorescence spect...More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetMicrotubule-associated protein tau(Human)
Oakland University

Curated by ChEMBL
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 2.20E+3nMAssay Description:Inhibition of His-tagged human brain tau 3R MBD aggregation after 16 hrs by thioflavin T fluorescence methodMore data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetIsoform Tau-F of Microtubule-associated protein tau(Human)
The Ohio State University

LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 610nMpH: 7.4Assay Description:The Tau content of fractions was determined by dot blot analysis using nitrocellulose membranes (0.2 μm porosity) as described previously [Cisek...More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
TargetPC4 and SFRS1-interacting protein(Human)
The Scripps Research Institute Molecular Screening Center

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 3.65E+4nMAssay Description:Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Affiliation: Ohio State University Assay Provider: Mam...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetFibrinogen beta chain [164-491](Human)
The Scripps Research Institute Molecular Screening Center

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 8.33E+4nMAssay Description:Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: Harvard Medical School/Massachuset...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetFibrinogen beta chain [164-491](Human)
The Scripps Research Institute Molecular Screening Center

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 9.25E+3nMAssay Description:Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: Harvard Medical School/Massachuset...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetPC4 and SFRS1-interacting protein(Human)
The Scripps Research Institute Molecular Screening Center

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.96E+3nMAssay Description:Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Affiliation: Ohio State University Assay Provider: Mam...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetApoptotic protease-activating factor 1(Human)
Burnham Center For Chemical Genomics

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.00E+5nMAssay Description:Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBIMR, San Diego, C...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetApoptotic protease-activating factor 1(Human)
Burnham Center For Chemical Genomics

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.55E+4nMAssay Description:Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBIMR, San Diego, C...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetCaspase-9(Human)
Burnham Center For Chemical Genomics

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 1.70E+4nMAssay Description:Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBIMR, San Diego, C...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetNeutrophil cytosol factor 1 [S99G](Human)
Emory University

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataIC50: 390nMAssay Description:NIH Molecular Libraries Screening Centers Network [MLSCN] Emory Chemical Biology Discovery Center in MLSCN Assay provider: Susan Smith, Emory Univers...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetZinc finger protein mex-5(Caenorhabditis elegans)
Broad Institute

Curated by PubChem BioAssay
LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataEC50:  1.14E+5nMAssay Description:Broad Institute: MLPCN maternal gene expression Project ID: 2024 Keywords: Zinc finger, C. elegans, maternal gene expression, RNA-protein interac...More data for this Ligand-Target Pair
In DepthDetails
PCBioAssay
TargetAcetylcholinesterase(Human)
Mayo Clinic

LigandPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
Affinity DataKi:  110nM ΔG°:  -9.49kcal/molepH: 7.0 T: 2°CAssay Description:Assays were conducted at 25 C in 20 mM sodium phosphate buffer (pH 7.0) and 0.01% bovine serum albumin unless otherwise noted. AChE concentrations we...More data for this Ligand-Target Pair
In DepthDetails Article
PubMed

Activity Spreadsheet -- ITC Data from BindingDB

Found 1 hit for monomerid = 50241461   

HostPNGBDBM197310(SAMPL5, CBClip)
University of California San Diego

GuestPNGBDBM50241461(Methylene Blue anhydrous | Methylenblau | bleu de ...)
ITC DataΔG°: -10.3kcal/mole logk: 3.90E+7
pH: 7.4 T: 24.85°C 
In DepthDetails Article
PubMed