TargetNEDD8-activating enzyme E1 catalytic/regulatory subunit(Human)
University of Macau
Curated by ChEMBL
University of Macau
Curated by ChEMBL
Affinity DataEC50: 3.40E+3nMAssay Description:Inhibition of NAE-mediated Ubcl2-NEDD8 conjugation in human Caco2 cells after 16 hrs by Western blot analysisMore data for this Ligand-Target Pair
Affinity DataEC50: 1.30E+3nMAssay Description:Inhibition of NAE-mediated Ubcl2-NEDD8 conjugation in human Caco2 cells after 16 hrs by Western blot analysisMore data for this Ligand-Target Pair
Affinity DataKd: 1.95E+5nMAssay Description:Binding affinity to biotin-labeled GST-tagged NAE (unknown origin) incubated for 1 hr by SPR assayMore data for this Ligand-Target Pair
Affinity DataEC50: 30nMAssay Description:Inhibition of NAE in human HCT-116 cells incubated for 1 hr by Western blot analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 0.0200nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 0.210nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 0.260nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 0.550nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 0.570nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 0.955nMAssay Description:Inhibition of recombinant human APPBP1/UBA3 expressed in Escherichia coli assessed as Ub/Ubl thioester transferMore data for this Ligand-Target Pair
Affinity DataIC50: 1nMAssay Description:Inhibition of NAE (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 2.20nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 2.30nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 3.60nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 4nMAssay Description:Inhibition of NAE1/UBA3 in human HCT-116 cells assessed as reduction in Ubc12-NEDD8 level incubated for 24 hrs by immunoblot analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 4nMAssay Description:Inhibition of NAE (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 4.20nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMAssay Description:Inhibition of human recombinant His-tagged NAE1 expressed in baculovirus expression system after 90 mins in presence of FLAG-tagged NEDD8 by TR-FRET ...More data for this Ligand-Target Pair
Affinity DataIC50: 5.80nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 6nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 6.10nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 8nMAssay Description:Inhibition of NAE in human HCT-116 cells incubated for 1 hr by Western blot analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 9.80nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Inhibition of NAE (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 16nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 23nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 32nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 32nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 64nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 64nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 67nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 81nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 82nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 91nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 102nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 107nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 114nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 114nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 120nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 122nMAssay Description:Inhibition of recombinant NAE (unknown origin) in presence of ATP at Km concentration by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 149nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 170nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
Affinity DataIC50: 187nMAssay Description:Inhibition of NAE (unknown origin) mediated neddylation assessed as decrease in NEDD8-Ubcl2 adduct formation incubated for 2 hrs in presence of L-glu...More data for this Ligand-Target Pair
