| Assay Method Information | |
| | Human STING WT Binding Assay |
| Description: | Cisbio Bioassays' human STING WT binding assay (#64BDSTGPEG & 64BDSTGPEH, Cisbio) is for quantitative measurement of human STING WT ligand using HTRF technology.1. Adding CompoundsNegative control: Dispense 5 μL of diluent into each negative control well. Standard: Dispense 5 μL of each Human STING WT Standard 2′3′-cGAMP (Std 0-Std 7) into each standard well. Compound: Dispense 5 μL of compound into each compound well.2. Adding ProteinsNegative control: Add 5 μL of detection buffer to all wells. Other wells: Add 5 μL of human STING WT protein 6His-tagged protein to all wells.3. Adding AntibodiesAdd 10 μL of premixed STING WT ligand d2 reagent and 6His Tb antibody working solution to all wells.4. RT IncubationSeal the plate and incubate 3 hours at RT or at Over Night if necessary.5. Reading PlateRemove the plate sealer and read on an HTRF compatible reader (PerkinElmer, USA). Results were analyzed with a two-wavelength signal ratio: intensity (665 nm)/intensity (620 nm).6. Curve FittingCalculate HTRF Ratio:Ratio=Signal665nmSignal620nm 104 |
| Affinity data for this assay | |
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