Reaction Details  Report a problem with these data
Cell Reactant:
CAM-BPTI
Syringe Reactant:
SecB
Meas. Tech.:
ITC
Entry Date.:
2000-12-13
ΔG°:
-29.4000±n/a (kJ/mole)
pH:
7.4000±n/a
Temperature:
281.1500±n/a (K)
ΔH° :
-25.2000±n/a (kJ/mole)
ΔCp :
-1.2180±n/a (kJ/mole)
Stoich. Param.:
1.0000
ΔS° :
-0.0149±n/a (kJ/mole-K)
Comments:
discrepancies in the fluorescence and calorimetric-derived binding constants
Citation
 Panse, VG; Swaminathan, CP; Surolia, A; Varadarajan, R Thermodynamics of substrate binding to the chaperone SecB. Biochemistry 39:2420-7 (2000) [PubMed]  Article
Cell React
Name:
Pancreatic trypsin inhibitor
Synonyms:
BPT1_BOVIN | CAM-BPTI | Aprotinin
Type:
Protein
Mol. Mass.:
10909.70
Organism:
Bovine
Description:
P00974
Residue:
100
Sequence:
MKMSRLCLSVALLVLLGTLAASTPGCDTSNQAKAQRPDFCLEPPYTGPCKARIIRYFYNAKAGLCQTFVYGGCRAKRNNFKSAEDCMRTCGGAIGPWENL
  
Syringe React
Source:
Expressed in E. coli strain BL21 (DE3)
Purity:
99%
Prep. Method:
Fast flow Q-Sepharose column, and then a Sephacryl S-200 gel filtration column
Name:
SecB
Synonyms:
n/a
Type:
Protein
Mol. Mass.:
17264.58
Organism:
Shigella flexneri serotype 5b (strain 8401)
Description:
Q0SYD5
Residue:
155
Sequence:
MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDLDTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYCPNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA