Compile Data Set for Download or QSAR
Report error Found 2492 of ic50 data for polymerid = 7285
LigandPNGBDBM50464991(CHEMBL4283739)
Affinity DataIC50: 0.100nMAssay Description:Inhibition of recombinant full length human GST-tagged ASK1 expressed in baculovirus expression system using STK3 peptide substrate preincubated for ...More data for this Ligand-Target Pair
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PubMed
LigandPNGBDBM443131(US10654833, Compound 5)
Affinity DataIC50: 0.300nMAssay Description:Inhibition of ASK1 kinase activity was determined radiometrically using 33P substrate incorporation (Reaction Biology Corp., Malvern, Pa.). Briefly, ...More data for this Ligand-Target Pair
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LigandPNGBDBM50464987(CHEMBL4290518)
Affinity DataIC50: 0.320nMAssay Description:Inhibition of recombinant full length human GST-tagged ASK1 expressed in baculovirus expression system using STK3 peptide substrate preincubated for ...More data for this Ligand-Target Pair
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PubMed
LigandPNGBDBM523902(US11136325, Compound 4 | 2-amino-N-[1-(5-chloro-7H...)
Affinity DataIC50: 0.398nMAssay Description:ASK1 and ASK2 biochemical activities were also quantified using AlphaScreen technology which measures the degree of phosphorylation of a protein subs...More data for this Ligand-Target Pair
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US Patent

LigandPNGBDBM187363(US9169243, 11 | US9908875, 11)
Affinity DataIC50: 0.400nMAssay Description:The assay measures the phosphorylation level of a biotinylated peptide substrate by the ASK1 kinase using HTRF detection. This is a competitive, time...More data for this Ligand-Target Pair
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LigandPNGBDBM50464992(CHEMBL4294318)
Affinity DataIC50: 0.420nMAssay Description:Inhibition of recombinant full length human GST-tagged ASK1 expressed in baculovirus expression system using STK3 peptide substrate preincubated for ...More data for this Ligand-Target Pair
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PubMed
LigandPNGBDBM483388(US10919911, Example 10)
Affinity DataIC50: 0.5nMAssay Description:Base Reaction buffer: 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO4, 2 mM DTT, and 1% DMSO were used for ...More data for this Ligand-Target Pair
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LigandPNGBDBM483415(US10919911, Example 37)
Affinity DataIC50: 0.5nMAssay Description:Base Reaction buffer: 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO4, 2 mM DTT, and 1% DMSO were used for ...More data for this Ligand-Target Pair
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LigandPNGBDBM483418(US10919911, Example 40)
Affinity DataIC50: 0.5nMAssay Description:Base Reaction buffer: 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO4, 2 mM DTT, and 1% DMSO were used for ...More data for this Ligand-Target Pair
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LigandPNGBDBM483400(US10919911, Example 22)
Affinity DataIC50: 0.600nMAssay Description:Base Reaction buffer: 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO4, 2 mM DTT, and 1% DMSO were used for ...More data for this Ligand-Target Pair
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LigandPNGBDBM483416(US10919911, Example 38)
Affinity DataIC50: 0.600nMAssay Description:Base Reaction buffer: 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO4, 2 mM DTT, and 1% DMSO were used for ...More data for this Ligand-Target Pair
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LigandPNGBDBM2579(CHEMBL388978 | Staurosporine, 8 | Staurosporin, 4 ...)
Affinity DataIC50: 0.690nMAssay Description:Inhibition of ASK1 (unknown origin) by HTRF assayMore data for this Ligand-Target Pair
In DepthDetails Article
PubMed
LigandPNGBDBM162322(US9051313, 13 | US10307427, Compound 13)
Affinity DataIC50: 0.700nMAssay Description:The assay measures the phosphorylation level of a biotinylated peptide substrate by the ASK1 kinase using HTRF detection (6.1). This is a competitive...More data for this Ligand-Target Pair
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LigandPNGBDBM50245498(CHEMBL4083562)
Affinity DataIC50: 0.700nMAssay Description:Inhibition of recombinant human ASK1 using STK3 peptide as substrate incubated for 30 mins followed by ATP addition measured for 3 hrs by TR-FRET ass...More data for this Ligand-Target Pair
In DepthDetails Article
PubMed
LigandPNGBDBM162322(US9051313, 13 | US10307427, Compound 13)
Affinity DataIC50: 0.700nMAssay Description:The assay measures the phosphorylation level of a biotinylated peptide substrate by the ASK1 kinase using HTRF detection (6.1). This is a competitive...More data for this Ligand-Target Pair
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LigandPNGBDBM443190(US10654833, Compound 102)
Affinity DataIC50: 0.700nMAssay Description:Inhibition of ASK1 kinase activity was determined radiometrically using 33P substrate incorporation (Reaction Biology Corp., Malvern, Pa.). Briefly, ...More data for this Ligand-Target Pair
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LigandPNGBDBM187354(US9169243, 2 | US9908875, 2)
Affinity DataIC50: 0.900nMAssay Description:The assay measures the phosphorylation level of a biotinylated peptide substrate by the ASK1 kinase using HTRF detection. This is a competitive, time...More data for this Ligand-Target Pair
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LigandPNGBDBM483401(US10919911, Example 23)
Affinity DataIC50: 0.900nMAssay Description:Base Reaction buffer: 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO4, 2 mM DTT, and 1% DMSO were used for ...More data for this Ligand-Target Pair
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LigandPNGBDBM483421(US10919911, Example 43)
Affinity DataIC50: 0.900nMAssay Description:Base Reaction buffer: 20 mM Hepes (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na3VO4, 2 mM DTT, and 1% DMSO were used for ...More data for this Ligand-Target Pair
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LigandPNGBDBM50464985(CHEMBL4282644)
Affinity DataIC50: 1nMAssay Description:Inhibition of recombinant full length human GST-tagged ASK1 expressed in baculovirus expression system using STK3 peptide substrate preincubated for ...More data for this Ligand-Target Pair
Ligand InfoPC cidPC sid
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PubMed
LigandPNGBDBM445505(US10683289, Example 22)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue # PV4011), ATP was purchased from Sigma (Catalogue # A7699), HTRF KinEASE Assay System was obtained f...More data for this Ligand-Target Pair
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LigandPNGBDBM445429(US10683279, Example 26a | US10988458, Example 26a ...)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher, ATP was purchased from Sigma , HTRF KinEASE Assay System was obtained from Cisbio (Bedford, Mass). Area plate w...More data for this Ligand-Target Pair
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LigandPNGBDBM445485(US10683289, Example 8)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue # PV4011), ATP was purchased from Sigma (Catalogue # A7699), HTRF KinEASE Assay System was obtained f...More data for this Ligand-Target Pair
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LigandPNGBDBM445488(US10683289, Example 10)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue # PV4011), ATP was purchased from Sigma (Catalogue # A7699), HTRF KinEASE Assay System was obtained f...More data for this Ligand-Target Pair
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LigandPNGBDBM445496(US10683289, Example 16)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue # PV4011), ATP was purchased from Sigma (Catalogue # A7699), HTRF KinEASE Assay System was obtained f...More data for this Ligand-Target Pair
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LigandPNGBDBM445499(US10683289, Example 18)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue # PV4011), ATP was purchased from Sigma (Catalogue # A7699), HTRF KinEASE Assay System was obtained f...More data for this Ligand-Target Pair
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LigandPNGBDBM445504(US10683289, Example 21)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue # PV4011), ATP was purchased from Sigma (Catalogue # A7699), HTRF KinEASE Assay System was obtained f...More data for this Ligand-Target Pair
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LigandPNGBDBM419047(US10450301, Example 126)
Affinity DataIC50: 1nMAssay Description:HTRF KinEASE-STK is a generic method for measuring serine/threonine kinase activities using a time-resolved fluorescence resonance energy transfer (T...More data for this Ligand-Target Pair
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LigandPNGBDBM419108(US10450301, Example 187)
Affinity DataIC50: 1nMAssay Description:HTRF KinEASE-STK is a generic method for measuring serine/threonine kinase activities using a time-resolved fluorescence resonance energy transfer (T...More data for this Ligand-Target Pair
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LigandPNGBDBM445429(US10683279, Example 26a | US10988458, Example 26a ...)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM497489(US11008304, Example 75)
Affinity DataIC50: 1nMAssay Description:The ability (IC50) of compounds to inhibit ASK1 kinase activity was determined by HTRF KinEASE Assay System. ASK1 was purchased from Thermofisher (Ca...More data for this Ligand-Target Pair
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LigandPNGBDBM497490(US11008304, Example 76)
Affinity DataIC50: 1nMAssay Description:The ability (IC50) of compounds to inhibit ASK1 kinase activity was determined by HTRF KinEASE Assay System. ASK1 was purchased from Thermofisher (Ca...More data for this Ligand-Target Pair
Ligand InfoPC cidPC sid
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LigandPNGBDBM497491(US11008304, Example 77)
Affinity DataIC50: 1nMAssay Description:The ability (IC50) of compounds to inhibit ASK1 kinase activity was determined by HTRF KinEASE Assay System. ASK1 was purchased from Thermofisher (Ca...More data for this Ligand-Target Pair
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LigandPNGBDBM497494(US11008304, Example 80)
Affinity DataIC50: 1nMAssay Description:The ability (IC50) of compounds to inhibit ASK1 kinase activity was determined by HTRF KinEASE Assay System. ASK1 was purchased from Thermofisher (Ca...More data for this Ligand-Target Pair
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LigandPNGBDBM523870(US11136324, Example 10 | 5-(4-Cyclopropyl-1H-imida...)
Affinity DataIC50: 1nMAssay Description:In this test, the fluorescence-resonance energy transfer (TR-FRET) method was used to determine the inhibition effect of the test compounds on ASK1 k...More data for this Ligand-Target Pair
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LigandPNGBDBM50579557(CHEMBL4868362)
Affinity DataIC50: 1nMAssay Description:Inhibition of ASK1 (unknown origin) using myelin basic protein as substrate preincubated for 20 mins followed by 33P-ATP addition and measured after ...More data for this Ligand-Target Pair
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PubMed
LigandPNGBDBM445429(US10683279, Example 26a | US10988458, Example 26a ...)
Affinity DataIC50: 1nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue # PV4011), ATP was purchased from Sigma (Catalogue # A7699), HTRF KinEASE Assay System was obtained f...More data for this Ligand-Target Pair
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LigandPNGBDBM523904(US11136325, Compound 6 | 2-amino-N-[1-(5-cyano-3-m...)
Affinity DataIC50: 1.05nMAssay Description:IMAP technology provides a homogeneous assay applicable to a wide variety of kinases, phosphatases, and phosphodiesterases without regard for substra...More data for this Ligand-Target Pair
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LigandPNGBDBM523904(US11136325, Compound 6 | 2-amino-N-[1-(5-cyano-3-m...)
Affinity DataIC50: 1.05nMAssay Description:ASK1 and ASK2 biochemical activities were also quantified using AlphaScreen technology which measures the degree of phosphorylation of a protein subs...More data for this Ligand-Target Pair
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US Patent

LigandPNGBDBM187369(US9169243, 17 | US9908875, 17)
Affinity DataIC50: 1.10nMAssay Description:The assay measures the phosphorylation level of a biotinylated peptide substrate by the ASK1 kinase using HTRF detection. This is a competitive, time...More data for this Ligand-Target Pair
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LigandPNGBDBM187385(US9169243, 33 | US9908875, 33)
Affinity DataIC50: 1.20nMAssay Description:The assay measures the phosphorylation level of a biotinylated peptide substrate by the ASK1 kinase using HTRF detection. This is a competitive, time...More data for this Ligand-Target Pair
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LigandPNGBDBM556127(US11345699, Example 12)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM556129(US11345699, Example 14)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM556130(US11345699, Example 15)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM556133(US11345699, Example 18)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM556134(US11345699, Example 19)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM556135(US11345699, Example 20)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM556137(US11345699, Example 22)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM556138(US11345699, Example 23)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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LigandPNGBDBM556141(US11345699, Example 26)
Affinity DataIC50: 1.25nMAssay Description:ASK1 was purchased from Thermofisher (Catalogue #PV4011), ATP was purchased from Sigma (Catalogue #A7699), HTRF KinEASE Assay System was obtained fro...More data for this Ligand-Target Pair
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